Lab meeting 12/6/2017

Kenny

Kruppert, S., Horstmann, M., Weiss, L. C., Schaber, C. F., Gorb, S. N. and Tollrian, R. (2016), Push or Pull? The light-weight architecture of the Daphnia pulex carapace is adapted to withstand tension, not compression. Journal of Morphology, 277: 1320–1328. doi:10.1002/jmor.20577

Comments: This paper is interesting because to has great images on the carapace of the daphnia. They went into detail about the structure and its significance why the “pillars” are shaped the way and how they used pressure to backed their hypothesis. Many staining techniques were used in this paper to identify the integument layers, which could be useful in the future of the lab.

Swatantra

Svendsen, N., Reisser, C. M. O., Dukić, M., Thuillier, V., Ségard, A., Liautard-Haag, C., … Haag, C. R. (2015). Uncovering cryptic asexuality in daphnia magna by RAD sequencing. Genetics, 201(3), 1143–1155. https://doi.org/10.1534/genetics.115.179879

Comments: This paper claims to provide the first demonstration of automictic parthenogenesis in Daphnia magna. Also, this paper shows the importance of heterozygosity patterns among offspring to elucidate the mode of reproduction in species. The supplemental data and protocol could be useful in future work along with the possibility to reproduce the result if desired.

Sen

Zong, C.; Lu, S.; Chapman, A.R.; Xie, S. (2012). “Genome-wide detection of single-nucleotide and copy-number variations of a single human cell.” Science 338, 1622. DOI: 10.1126/science.1229164

Comments: Original paper describing the MALBAC whole gneome amplification protocol

 

Marelize

Elena Gorokhova, Marcia Kyle; Analysis of nucleic acids in Daphnia: development of methods and ontogenetic variations in RNA-DNA content, Journal of Plankton Research, Volume 24, Issue 5, 1 May 2002, Pages 511–522https://doi.org/10.1093/plankt/24.5.511

Comments: I found this paper to be interesting because it analyzed how the DNA and RNA content changed during the developmental stages of Daphnia. The paper also discussed how RNA/DNA concentrations are related to changes in metabolic activity associated with development.

Trung

Gallegos, J. E. & Rose, A. B. The enduring mystery of intron-mediated enhancement. Plant Science 237, 8–15 (2015).

Comments: Good review article that may help us narrow our search into introns of interest that affect gene expression in Daphnia.

Skip to toolbar