Rahul Kumar / Psychology / Faculty Mentor: Yuan B Peng
The current study investigates Phase-Amplitude Coupling (PAC) across four corresponding brain regions consisting of the Anterior Cingulate Cortex (ACC), Right Amygdala (RAMG), Left Amygdala (LAMG), and Ventral Tegmental Area (VTA) under different physiological states: light isoflurane (1.5%) from 0-2 minutes, deep isoflurane (5%) from 2-3 minutes, no isoflurane from 3-8 minutes, followed by euthanasia by CO₂ exposure from 8-18 minutes. PAC was quantified using the Modulation Index (MI) with a bandpass filter approach on Local Field Potential (LFP) recordings segmented into 30-second epochs. Statistical analysis of MI (mean +/- SEM) were assessed across these states. The results reveal a trend of progressive suppression of PAC with increasing isoflurane levels in some recorded regions, followed by a rise in pre-CO₂, and decrease in post-CO₂ exposure. However, no significant PAC differences were found across the states, probably due to a limited sample size (n=5). These findings have the potential to offer insights into how anesthesia and euthanasia alter brain oscillations, providing an alternative approach to understand brain function and consciousness.” modalities.
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